I really wouldn't recommend using FMU (first morning urine) beacause of the way LH (what the OPK detects) is metabolised. The recommended time for testing is the afternoon, from around 2pm til 6pm ish; from a website, hope this helps
Tip 4: OPKs in the Afternoon.
Unlike pregnancy tests, ovulation predictor kits should not be used with first morning urine. LH - or luteinizing hormone is - is synthesized by the body in the morning, but it does not show up in your system (urine) until afternoon. Most manufacturers recommend taking ovulation tests between 10AM and 8PM - though afternoon is considered the ideal testing time. Also, it is recommended that you test at the same time every day
FYI - I never time how long I hold it for just make sure I dont drink excessively from lunchtime
