for the first time i use a OPK from first response any way in the how to use booklet they said once you have you positive 2 dark lines you should ovulate in the next 24 h to 36 hours. i did mine on wednesday .thursday and i got the dark lines on friday

but the booklet said that once you have the positive you can stop the testing (and i did and went on with my BD session)
anyway my question is if i was going to use a opk test again tomorrow what will i see? i mean does you "O" hormones disappear and you can not detect them after that?
i was just wondering?